The Johnstone lab studies regulation of aqueous outflow in glaucomas through studies of tissue biomechanics and related morphology.
The Johnstone lab studies regulation of aqueous outflow in glaucoma. Studies of tissue biomechanics involve subjecting aqueous outflow system tissues and fluids to loading forces induced by changing pressure relationships, then observing resulting tissue deformation. Morphologic studies use brightfield microscopy of 1-µm plastic embedded sections, SEM and TEM. Tissues constituents and relationships are further studied by immunohistochemistry techniques; 3D images are obtained by confocal microscopy and serial registered 1µ sections followed by FIJI, Imaris and Resolution technology 3D projections.